FMD Situation in Bulgaria, 2010-2011
Valdazo-Gonzalez, Nick J. Knowles, Jef
Hammond and Donald King
OIE/FAO/Defra Reference Laboratory for
FMD (WRLFMD), Institute for Animal
Health, Pirbright, United Kingdom.
The FAO/OIE/Defra Reference Laboratory
for FMD at the Institute for Animal
Health (UK) has determined the full
genome sequence for a representative
serotype O FMDV isolate (O/BUL/1/2010)
recovered from the recent outbreaks of
FMD that have occurred in Bulgaria.
Since the end of December 2010 three
cases of FMD have been reported in
south-eastern Bulgaria, close to the
border with Turkish Thrace. VP1 sequence
analysis on samples received from the
first case of FMD in Bulgaria in a wild
boar was carried out at the IAH and
showed that the virus belongs to the
MIDDLE EAST-SOUTH ASIA (ME-SA) topotype
(PanAsia-2 lineage, ANT-10 sub-lineage)
and is closely related to viruses found
in Turkey and Iran (sharing nucleotide
identities of >99 percent;
Our studies have shown that the ANT-10
sub-lineage of the PanAsia-2 lineage
(which was derived from the earlier
PanAsia lineage) has recently become
widespread in Afghanistan, Iran,
Pakistan and Turkey.
A full genome sequence (8180
nucleotides, excluding the poly-A tail)
was generated from viral RNA extracted
from an epithelial lesion collected from
a wild boar that was shot in the Burgas
region of Bulgaria on 30 Dec 2010. The
available as a draft GenBank submission.
The full genome sequence of a closely
related virus isolated from an outbreak
in Turkey is currently being determined
at the IAH for comparative purposes.
During previous FMD outbreaks in the UK
(2001 and 2007), full genome sequence
data has been used to reconstruct
transmission pathways at the
farm-to-farm level: for examples see
Cottam et al. (2006) and Cottam et al.
(2008). This approach could now be used
to support epidemiological
investigations that are being conducted
to understand the spread of the virus
to, and within, Bulgaria.
Further information, including detailed
laboratory RT-PCR protocols used to
generate this sequence, can be obtained
from the WRLFMD:
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Š The Pirbright Institute, 2011